74DEVELOPMENT AND VALIDATION OF AN ANALYTICAL METHOD FOR THE SIMULTANEOUS ESTIMATION OF SUMATRIPTAN AND NAPROXEN BY RP-HPLCIn the present work a simple, sensitive and stability indicating Reverse Phase High Performance Liquid Chromatography (RP-HPLC) method has been developed and validated for the determination of Sumatriptan and Naproxen in Bulk and Pharmaceutical Dosage form to create an affordable and easy method than the previous one. The work was executed on SHIMADZU instrument in isocratic mode, with mobile phase containing methanol: phosphate buffer (pH 3.0) in the ratio of 70:30 is used. The flow rate is 1.0ml/min, and analytes are monitored at 278 nm. Chromatogram showed peaks at a retention time of 2.150min for Sumatriptan& 3.751min for Naproxen. The method is validated for system suitability, linearity, precision, accuracy, specificity, robustness, LOD and LOQ. Recovery of Sumatriptan and Naproxen was found to be in the range of 98.18 – 100.89 %. The correlation coefficient of Sumatriptan and naproxen were found to be 0.997 & 0.996. The %RSD for repeatability was found to be 0.8 & 0.38. LOD and LOQ for the estimation of Sumatriptan and Naproxen are found to be 2.90 & 2.97, 9.72 & 9.86 respectively. Proposed method can be successfully applied for the quantitative determination of Sumatriptan and Naproxen in bulk drug and Tablet dosage formsunilpadavala@gmail.comResearch512015Naproxen,Sumatriptan,RP-HPLCP.Sudha Rani,P. Sunil Kumar Chaitanya,G. RohiniReddy,P.MuraliDepartment of Pharmaceutical Analysis & Quality Assurance, St. Pauls College of Pharmacy, Turkayamjal, Hyderabad, India,Department of Pharmaceutical Analysis & Quality Assurance, St. Pauls College of Pharmacy, Turkayamjal, Hyderabad, India,Department of Pharmaceutical Analysis & Quality Assurance, St. Pauls College of Pharmacy, Turkayamjal, Hyderabad, India,Department of Pharmaceutical Analysis & Quality Assurance, St. Pauls College of Pharmacy, Turkayamjal, Hyderabad, India