57PHARMACOGNOSTIC, PHYTOCHEMICAL AND PHYSICOCHEMICAL INVESTIGATION OF CROTON TIGLIUM SEEDSBackground: Jayapala/Jepala (Croton tiglium Linn.) belongs to the family Euphorbiaceae. The croton plant is a native of India and is grown all through the East Indies. The seeds are poisonous and contain crotin, a toxalbumin. The drug material consisting seeds are used in therapeusis after proper purification (Shodhana) and with posological consideration in Ayurvedic pharmaceutics. Aims and objectives: The present study was carried out to verify the pharmacognostical identity of the sample and to carry phytochemical and physicochemical investigations of Croton tiglium seed. Methods and materials: Pharmacognostical investigations were carried out to study its macro and microscopical characters. Phytochemical parameters like estimation of the croton oil and various physiochemical parameters like pH, moisture content, ash value, extractives values and HPTLC etc. were carried out. Results: The transverse section of the seed revealed the presence of aleurone grains, epidermis of cotyledon, epidermis of testa, rosette crystals of calcium oxalate, sclereids and oil cells. The results of physiochemical parameters showed pH - 6.03%w/w, moisture content - 3.92% w/w, total ash - 2.80% w/w, acid insoluble ash - 0.50%w/w, water soluble extractive - 7.64%w/w and alcohol soluble extractive - 29.16%w/w. and HPTLC profile shows presence of 12 spots and 1 spot at 254 and 366 nm respectively. Conclusion: The given sample has been authenticated as seeds of Croton tiglium as the macro-microscopic features and the physicochemical parameters mentioned for Jayapala in API (Ayurvedic Pharmacopoeia of India) matches with that of test sampledrjyotibaragi@gmail.comResearch432014Croton tiglium,Pharmacognosy,Phytochemical,Physico-chemicalJyoti M Ganer,Vijay V Nikam,Baragi Umapati C,Baragi Pramod CP.G. Scholar,Dept. of Agada Tantra, L.R.P. Ayurvedic Medical College, P.G. Institute Research Center, Islampur, Sangli, Maharashtra, India,Professor, Dept. of Agada Tantra, L.R.P. Ayurvedic Medical College, P.G. Institute Research Center, Islampur, Sangli, Maharashtra, India, Assistant Professor, Dept. of Basic Principles, S.D.M. College of Ayurveda, Udupi, Karnataka, India,Associate Professor, Dept. of Rasashastra and Bhaishajya Kalpana, N. K. Jabshetty Ayurved Medical College and Post Graduate Centre, Bidar, Karnataka, India